書籍名 |
テロメア・テロメラーゼ |
出版社 |
日本医学館
|
発行日 |
1999-07-30 |
著者 |
|
ISBN |
4890444130 |
ページ数 |
269 |
版刷巻号 |
第1版第1刷 |
分野 |
|
閲覧制限 |
未契約 |
第一線研究者によるわかりやすい概説と臨床指針。
テロメア,テロメラーゼ研究の決定版。
目次
- 表紙
- 執筆者一覧
- 序
- 目次
- 序論
- テロメア・テロメラーゼ研究の進歩
- I. 基礎
- 1. ヒト細胞の老化と不死化
- ヒト体細胞には分裂寿命がある
- 細胞分裂のたびにテロメアDNAが短縮する
- テロメアは分裂時計である
- 遺伝的早老症とテロメア代謝異常
- 活性酸素とテロメア代謝異常
- 分裂寿命がつきた細胞の増殖停止の仕組み
- 細胞老化を表すシグナル伝達プロセス
- 遺伝的早老症の原因遺伝子の機能
- 分裂寿命の延長は容易であるが不死化は難しい
- Bリンパ球も容易には不死化しない
- 細胞の不死化
- テロメラーゼ
- ヒト培養細胞のテロメラーゼ活性
- ヒト組織のテロメラーゼ活性
- テロメラーゼの発現によって細胞は不死化するか
- 細胞の老化とヒトの老化
- 2. テロメア・テロメラーゼの構造と機能
- テロメアの構造と機能
- テロメラーゼは鋳型RNAを内在する逆転写酵素である
- テロメラーゼ逆転写酵素: テロメラーゼの触媒蛋白質サブユニット
- その他のテロメラーゼ付属蛋白質(telomerase-associated protein)
- テロメアに作用するものはテロメラーゼだけではない
- 3. テロメラーゼ活性制御因子とテロメア結合蛋白
- サブユニット蛋白・RNAの発現調節を介するテロメラーゼ酵素活性の制御
- ヒト3番染色体短腕(3p)に存在する細胞老化誘導/テロメラーゼリプレッサー遺伝子
- テロメアDNA結合蛋白
- 4. リンパ細胞におけるテロメラーゼ誘導シグナル
- テロメラーゼ活性を誘導するシグナルとその細胞内伝達経路
- シグナルの最終ターゲットは何か
- なぜリンパ細胞の活性化に伴い, テロメラーゼ活性も誘導されるのか
- 5. 正常上皮細胞のテロメラーゼ活性と幹細胞
- 正常細胞のテロメラーゼ
- 正常上皮細胞のテロメラーゼ活性の強さ
- テロメラーゼ活性が検出される正常組織
- 正常細胞のテロメラーゼ活性の特性
- 上皮組織を構成する細胞サブセット
- すべての基底層細胞がテロメラーゼ活性をもつわけではない
- テロメラーゼ活性をもたない細胞サブセット
- II. 測定法
- 6. テロメア長測定
- 7. テロメアのin situによる検出方法(FISH/PRINS)
- ペプチド核酸によるFISH法を用いたテロメア配列の検出
- Cycling PRINS法
- FISH/PRINS法の実際
- 8. テロメラーゼ活性測定法
- a. TRAPアッセイ
- b. Non-RIによるテロメラーゼ活性の検出法
- PCR-ELISA法
- TRAP-HPA法
- F-TRAP法
- c. In situ telomeric repeat amplification protocol(TRAP)法
- In situ TRAP法
- In situ TRAP法の実際
- 組織 in silu TRAP
- d. Stretch PCR法
- Stretch PCR法の特徴
- Stretch PCR法のプロトコール
- Stretch PCR法の実際
- Stretch PCR法とTRAP法の比較
- Stretch PCR法の問題点
- 9. テロメア, テロメラーゼ関連遺伝子の検出
- AGPC法を用いたRT-PCR grade RNAの調整法
- RT-PCR法によるテロメラーゼ関連遺伝子の検出
- III. ヒト正常細胞・癌細胞のテロメア長・テロメラーゼ活性
- 10. 早老症とテロメア
- 早老症
- ウェルナー症候群とプロジェリア
- 早老症細胞の分裂寿命とテロメア短縮, テロメラーゼ
- 早老症遺伝子と分裂寿命とテロメアの異常
- 糖尿病, 高血圧とテロメア短縮
- 11. リンパ球・免疫疾患のテロメア長・テロメラーゼ活性
- リンパ球のテロメア長
- 末梢血リンパ球のテロメラーゼ活性
- 組織中のリンパ球テロメラーゼ活性
- リンパ球のテロメラーゼ活性はテロメア長を安定に保てるか?
- 免疫疾患のテロメラーゼ活性
- 12. 造血幹細胞, 造血器腫瘍のテロメア長,テロメラーゼ
- 造血幹細胞のテロメラーゼ動態
- 造血細胞のテロメア動態
- 正常人末梢血単核球でのテロメア長とテロメラーゼ活性
- 急性白血病のテロメア
- 急性白血病のテロメラーゼ
- 慢性骨髄性白血病のテロメア動態
- 骨髄異形成症候群におけるテロメラーゼ活性
- 13. 肺癌のテロメア長・テロメラーゼ活性
- 肺癌原発巣におけるテロメア長・テロメラーゼ活性
- 肺癌転移巣におけるテロメア長・テロメラーゼ活性
- 前癌病変におけるテロメラーゼ活性・hTR発現
- テロメラーゼ活性を指標とした肺癌の存在診断
- テロメラーゼ活性を指標とした肺癌の生物学的特性の診断
- テロメラーゼをターゲットとした抗癌戦略への期待
- 14. 消化管・消化器腫瘍のテロメア長・テロメラーゼ
- 正常の消化管のテロメア, テロメラーゼ
- 消化管由来の癌におけるテロメア長とテロメラーゼ活性
- 消化器実質臓器のテロメラーゼ活性
- 15. 膀胱癌, 乳癌におけるテロメラーゼ活性とその臨床応用
- 材料と方法
- 膀胱癌でのテロメラーゼ活性
- 乳癌におけるテロメラーゼ活性
- テロメラーゼインヒビターは存在するか
- 臨床応用の可能性
- 16. 皮膚・毛包・皮膚腫瘍のテロメラーゼ活性
- 正常皮膚でのテロメラーゼ活性
- テロメラーゼ陽性の基底細胞は幹細胞か
- 日光曝露部皮膚でのテロメラーゼ活性
- 口光曝露部皮膚でのテロメラーゼ活性とp53遺伝子変異
- 毛包におけるテロメラーゼ
- ヒト皮膚腫瘍におけるテロメラーゼ活性
- テロメラーゼ・サブユニットの発現と皮膚腫瘍におけるテロメラーゼ活性との関連
- 皮膚におけるテロメラーゼ活性の制御
- 17. 婦人科組織のテロメラーゼ活性
- 婦人科腫瘍のテロメラーゼ活性
- 前癌, 良性病変のテロメラーゼ活性
- 癌の臨床病理学的特徴とテロメラーゼ活性の関係
- 子宮内膜のテロメラーゼ活性
- 絨毛組織のテロメラーゼ活性
- テロメラーゼ活性の測定による癌診断の可能性
- テロメラーゼサブユニットの発現
- テロメラーゼ活性の生物学的意義
- 18. 小児固形腫瘍のテロメア長・テロメラーゼ
- 発生とテロメア・テロメラーゼ
- 神経芽細胞腫のテロメア・テロメラーゼ
- 網膜芽細胞腫のテロメア・テロメラーゼ
- 腎芽腫のテロメア・テロメラーゼ
- 肝芽腫のテロメア・テロメラーゼ
- 奇形腫のテロメア・テロメラーゼ
- その他の小児固形腫瘍のテロメア・テロメラーゼ
- 小児固形悪性腫瘍のテロメア・テロメラーゼの特徴
- 19. 頭頚部腫瘍, 甲状腺腫瘍, 脳腫瘍, その他の腫瘍/組織
- 頭頚部癌
- 甲状腺腫瘍
- 副腎腫瘍
- 骨軟部腫瘍/組織
- 脳腫瘍
- 前立腺腫瘍
- 腎腫瘍
- IV. 新薬開発
- 20. テロメア・テロメラーゼをターゲットとした新薬開発
- テロメラーゼ阻害剤の開発
- テロメア機能の阻害剤
- テロメア・テロメラーゼ機能阻害剤の問題点
- テロメラーゼ発現による分裂寿命延長の試み
- おわりに
- 略語
- キーワード
- 索引
- 奥付
参考文献
序論
P.6 掲載の参考文献
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II. 測定法
P.86 掲載の参考文献
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P.99 掲載の参考文献
P.103 掲載の参考文献
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P.116 掲載の参考文献
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1) Tatematsu K, Nakayama J, Danbara M, Shionoya S, Sato H, et al.:A novel quantitative stretch PCR assay that detects a dramatic increase in telomerase activity during the progression of myeloid leukemias. Oncogene 13:2265-2274, 1996.
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III. ヒト正常細胞・癌細胞のテロメア長・テロメラーゼ活性
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P.161 掲載の参考文献
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P.167 掲載の参考文献
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P.177 掲載の参考文献
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P.183 掲載の参考文献
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P.189 掲載の参考文献
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2) Yasumoto S, Kunimura C, Kikuchi K, Tahara H, 0hji H, et al.:Telomerase activity in normal human epithelial cells. Oncogene 13:433-439, 1996.
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4) Ueda M, Ouhtit A, Bito T, Nakazawa K, Lubbe J, et al.:Evidence for UV-associated activation of telomerase in human skin. Cancer Res. 57:370-374, 1997.
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P.218 掲載の参考文献
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IV. 新薬開発
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