書籍名 |
分子生物学、細胞遺伝学、細胞免疫学に基づく 血液・固形腫瘍診断マニュアル 改訂版 |
出版社 |
フジメディカル出版
|
発行日 |
2002-10-01 |
著者 |
|
ISBN |
4939048195 |
ページ数 |
282 |
版刷巻号 |
第1版第1刷 |
分野 |
|
閲覧制限 |
未契約 |
腫瘍診断に欠かすことのできない、遺伝子診断法、染色体検査法、細胞免疫学的検査法、タンパク分析法について、その原理・手技から腫瘍ごとの診断法・治療への応用まで、最新知見がコンパクトに解説されています。
見やすさ、使いやすさがさらに充実した、待望の改訂版。癌臨床に必携の1冊。
目次
- 表紙
- 編者・執筆者一覧
- 序
- 目次
- 1. 細胞形態、細胞化学に基づく診断
- 2. 遺伝子診断法
- 1) ブロッティング法
- 2) 遺伝子増幅法
- ポリメラーゼ連鎖反応 (PCR)
- PCR法を用いた遺伝子変異の検出
- 遺伝子再構成の検出
- 点突然変異の検出
- (1) PCR-SSCP
- (2) 変異プライマー法
- (3) 変性濃度勾配ゲル電気泳動法
- PCRを用いたLOH解析
- PCRを用いたクローナリティ解析
- 定量的PCR法
- (1) competitive PCR法
- (2) リアルタイムPCR
- 3) DNAチップ法
- 3. 腫瘍の遺伝子検査と倫理
- 4. 染色体検査法
- 1) 分染法
- 2) FISH法、間期核FISH法
- 3) SKY法
- 5. 細胞免疫学的診断法
- 1) フローサイトメトリー
- 2) CD45ゲーティングを用いた解析
- 6. タンパク分析法
- 7. 検査用検体の処理と保存
- <カラー図譜>
- 8. 腫瘍の遺伝子・免疫学的診断と臨床的意義
- 1) 造血器腫瘍
- 急性骨髄性白血病
- 急性リンパ性白血病
- 慢性骨髄性白血病
- 成人T細胞白血病
- 悪性リンパ腫
- 骨髄腫
- 骨髄異形成症候群
- 2) 固形腫瘍
- 9. 遺伝子・免疫学的検査の治療への応用
- 1) 微小残存病変の診断
- 造血器腫瘍
- (1) PCR法を用いた診断
- (2) 細胞免疫学的手法を用いた診断
- 固形腫瘍のMRD
- 小児固形腫瘍 (神経芽腫、横紋筋肉腫、Ewing肉腫など)
- 2) 造血幹細胞移植における応用
- 3) 血液腫瘍に伴う感染症診断への応用
- 文献
- <参考資料>
- 染色体模式図
- 造血器腫瘍において染色体転座・逆位により再構成を受ける遺伝子一覧
- 細胞免疫診断に役立つモノクローナル抗体の一覧表
- 診断によく用いられる表面マーカーの組み合わせ例と分化度の評価
- 索引
- 奥付
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