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未契約
書籍名 分子生物学、細胞遺伝学、細胞免疫学に基づく 血液・固形腫瘍診断マニュアル 改訂版
出版社 フジメディカル出版
発行日 2002-10-01
著者
  • 横田昇平(編集)
ISBN 4939048195
ページ数 282
版刷巻号 第1版第1刷
分野
閲覧制限 未契約

腫瘍診断に欠かすことのできない、遺伝子診断法、染色体検査法、細胞免疫学的検査法、タンパク分析法について、その原理・手技から腫瘍ごとの診断法・治療への応用まで、最新知見がコンパクトに解説されています。 見やすさ、使いやすさがさらに充実した、待望の改訂版。癌臨床に必携の1冊。

目次

  • 表紙
  • 編者・執筆者一覧
  • 目次
  • 1. 細胞形態、細胞化学に基づく診断
  • 2. 遺伝子診断法
    P.12閲覧
    • 1) ブロッティング法
    • 2) 遺伝子増幅法
      • ポリメラーゼ連鎖反応 (PCR)
      • PCR法を用いた遺伝子変異の検出
        • 遺伝子再構成の検出
        • 点突然変異の検出
          • (1) PCR-SSCP
          • (2) 変異プライマー法
          • (3) 変性濃度勾配ゲル電気泳動法
      • PCRを用いたLOH解析
      • PCRを用いたクローナリティ解析
      • 定量的PCR法
        • (1) competitive PCR法
        • (2) リアルタイムPCR
    • 3) DNAチップ法
  • 3. 腫瘍の遺伝子検査と倫理
    P.76閲覧
  • 4. 染色体検査法
    P.78閲覧
    • 1) 分染法
    • 2) FISH法、間期核FISH法
    • 3) SKY法
  • 5. 細胞免疫学的診断法
    P.96閲覧
    • 1) フローサイトメトリー
    • 2) CD45ゲーティングを用いた解析
  • 6. タンパク分析法
    P.110閲覧
    • SDS-PAGE、ウェスタン法、免疫沈降
  • 7. 検査用検体の処理と保存
    P.118閲覧
    • 1) 造血器腫瘍 (血液・骨髄)
    • 2) 固形腫瘍
  • <カラー図譜>
    P.130閲覧
  • 8. 腫瘍の遺伝子・免疫学的診断と臨床的意義
    P.140閲覧
    • 1) 造血器腫瘍
      • 急性骨髄性白血病
      • 急性リンパ性白血病
      • 慢性骨髄性白血病
      • 成人T細胞白血病
      • 悪性リンパ腫
      • 骨髄腫
      • 骨髄異形成症候群
    • 2) 固形腫瘍
  • 9. 遺伝子・免疫学的検査の治療への応用
    P.202閲覧
    • 1) 微小残存病変の診断
      • 造血器腫瘍
        • (1) PCR法を用いた診断
        • (2) 細胞免疫学的手法を用いた診断
      • 固形腫瘍のMRD
        • 小児固形腫瘍 (神経芽腫、横紋筋肉腫、Ewing肉腫など)
    • 2) 造血幹細胞移植における応用
    • 3) 血液腫瘍に伴う感染症診断への応用
  • 文献
    P.238閲覧
  • <参考資料>
    P.257閲覧
    • 染色体模式図
    • 造血器腫瘍において染色体転座・逆位により再構成を受ける遺伝子一覧
    • 細胞免疫診断に役立つモノクローナル抗体の一覧表
    • 診断によく用いられる表面マーカーの組み合わせ例と分化度の評価
  • 索引
    P.272閲覧
  • 奥付

参考文献

1. 細胞形態、細胞化学に基づく診断

P.6 掲載の参考文献

  • 2) Shafer JA:Preparation of blood films for examination. in Clinical hematology Principles, procedures, correlations, Lippincott, Philadelphia, 1998

2. 遺伝子診断法

P.12 掲載の参考文献

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3. 腫瘍の遺伝子検査と倫理

P.78 掲載の参考文献

  • 1) 桜井雅温:白血病の染色体に関する国際ワークショップ. 腫瘍染色体アトラス (阿部達生ほか編), 南江堂, 東京, 1986, p147
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5. 細胞免疫学的診断法

P.96 掲載の参考文献

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6. タンパク分析法

P.110 掲載の参考文献

7. 検査用検体の処理と保存

P.118 掲載の参考文献

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8. 腫瘍の遺伝子・免疫学的診断と臨床的意義

P.140 掲載の参考文献

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9. 遺伝子・免疫学的検査の治療への応用

P.202 掲載の参考文献

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